Understand if your compound is metabolized by MAO-A or MAO-B enzyme.
Monoamine oxidase (MAO) reaction phenotyping is a non-CYP mediated metabolism assay within our portfolio of in vitro ADME screening services. Cyprotex deliver consistent, high quality data with the flexibility to adapt protocols based on specific customer requirements.
Determining potential metabolism by MAO enzymes
Monoamine oxidases (MAO) are membrane-associated enzymes located specifically on the outer mitochondrial membrane. They are the major enzymes participating in the catabolism of monoamine neurotransmitters and related exogenous amines.
Two isoforms of MAO exist, MAO-A and MAO-B, which differ in their substrate specificity, inhibitor selectivity and tissue distribution.
Selective MAO-A inhibitors are useful in the therapy of depression and anxiety whereas MAO-B inhibitors are often used in the treatment of Parkinson’s and Alzheimer’s diseases.
Cyprotex’s MAO reaction phenotyping assay identifies if your compound is a substrate for either MAO-A or MAO-B.
In human brain the predominant form is MAO-B, expressed at highest levels in astrocytes and serotonergic neurons, while MAO-A is expressed at highest levels in catecholaminergic neurons.
1Hotamisligil GS and Breakefield XO (1991) Am J Hum Genet49(2); 383–392